ENaC alpha Antibody: HRP

• Catalog Number: orb383759
• Category: Antibodies
• Description: Mouse monoclonal antibody against ENaC alpha conjugated to HRP.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2G4
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: IgG2b
• Immunogen: Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC (1:150)
• Conjugation: HRP
• MW: 85kDa
• Target: ENaC alpha
• Entrez: 25122
• UniProt ID: Q6IRJ1
• NCBI: NP_113736
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: SCNN1A antibody, Epithelial Sodium Channel-α antib
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-239 was sufficient for detection of ENaC alpha in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.