ENaC alpha Antibody: HRP

• Catalog Number: orb152719
• Category: Antibodies
• Description: Rabbit polyclonal antibody against ENaC Alpha conjugated to HRP
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Frog, Human, Mouse, Rat
• Immunogen: Produced against a synthetic peptide mapping to the N-terminal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:400); IHC (1:25)
• Conjugation: HRP
• MW: 85kDa
• Target: ENaC
• Entrez: 25122
• UniProt ID: Q6IRJ1
• NCBI: NP_113736
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: SCNN1A antibody, Epithelial Sodium Channel-α antib
• Note: For research use only
• Application notes: 1 µg/ml of SPC-403 was sufficient for detection of alpha-ENaC in 35 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) ENaC Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.