EN1/Engrailed Antibody

SKU: orb1538560

Description

EN1/Engrailed Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: ELISA, IHC, IHC-P (5 µg/ml), WB (1:1000)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot performed with the recombinant protein used as the immunogen.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone No.: 1F5
• Immunogen: EN1 (NP_001417, 266 aa ~ 392 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: EN1 / Engrailed
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EN1, Engrailed 1, Engrailed homolog 1, Homeobox protein en-1, Homeobox protein engrailed-1, Hu-En-1, Engrailed homeobox 1

Anti-EN1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Detection limit for recombinant GST tagged EN1 is approximately 1 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

EN1 / Engrailed