EN1 (Engrailed 1) Antibody (N-term)

SKU: orb37720

This product can be custom made

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Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-P, WB
Dilution Range	WB: 1:1000, IF/ICC: 1:10-50, IHC-P: 1:10-50
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	Synthetic Peptide
Target	EN1
Molecular Weight	40115
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-Homeobox protein engrailed-1 antibody, anti-Hu-En-1 antibody, anti-HME1 antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-EN1 (Engrailed 1) Antibody (N-term) at 1:2000 dilution. Lane 1: U-87 MG whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: 293T/17 whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (Human Sarcoma cell line) cells labeling HME1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Immunofluorescence image showing nucleus and nucleoli staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin at 1/100 dilution (red).

Fluorescent image of U251 cell stained with EN1 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). EN1 immunoreactivity is localized to Nucleus significantly.