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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:1000 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,K |
| Clone No. | B446EV8X7X5 |
| Immunogen | This ELAVL1 monoclonal antibody is generated from mouse immunized with ELAVL1 recombinant protein. |
| Target | ELAVL1 |
| Molecular Weight | 36092 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ELAV-like protein 1, Hu-antigen R, HuR, ELAVL1, HUR
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All lanes: Anti- at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Jurkat whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of ELAVL1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001410.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELAVL1 Antibody (orb1939371)
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