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ELAVL1 Antibody
SKU: orb1274490
Featured
Description
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| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2). |
| Target | ELAVL1 |
| Molecular Weight | Observed: 37kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG
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ELAVL1 Antibody [orb395161]
ELISA, FC, IF, IHC, IP, WB
Human, Mouse, Rat, Zebrafish
Rabbit
Polyclonal
Unconjugated
100 μg, 50 μg
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Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using HuR/ELAVL1 antibody (orb1274490). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
ELAVL1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELAVL1 Antibody (orb1274490)
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