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Catalog Number | orb1256571 |
---|---|
Category | Antibodies |
Description | ELAC2 Antibody |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ELAC2 (NP_776065.1). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 105kDa |
Target | ELAC2 |
UniProt ID | Q9BQ52 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | ELAC2, ElaC (E. coli) homolog 2, ElaC homolog 2 (E Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ELAC2 Antibody (orb1256571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human esophagus using ELAC2 antibody (orb1256571) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using ELAC2 antibody (orb1256571). Blue: DAPI for nuclear staining.
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |