Rabbit ELAC2 Antibody

SKU: orb1522355

Description

ELAC2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 150 and 200
• Target: ELAC2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COXPD17; elaC homolog 2; elaC homolog protein 2; elaC-like protein 2; ELC2; heredity prostate cancer protein 2; HPC2; putative prostate cancer susceptibility protein HPC2/ELAC2; ribonuclease Z 2; RNase Z 2; tRNA 3 endonuclease 2; tRNase Z (long form); tRNase Z 2; zinc phosphodiesterase ELAC protein 2

Detection of human ELAC2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ELAC2 antibody orb1522355 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ELAC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ELAC2 antibody orb1522355 used for IP at 6 µg per reaction.

Detection of human ELAC2 by immunohistochemistry.Sample: FFPE section of human stomach. Antibody: Affinity purified rabbit anti-ELAC2 (Cat. No. orb1522355) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse ELAC2 by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-ELAC2 (Cat. No. orb1522355) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

ELAC2

UniProt

Q9BQ52

RefSeq (Protein):NP_060597.4