EIF6 Antibody

• Catalog Number: orb1258453
• Category: Antibodies
• Description: EIF6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 27kDa
• Target: EIF6
• UniProt ID: P56537
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CAB, EIF3A, ITGB4BP, b(2)gcn, eIF-6, p27(BBP), p27
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EIF6 antibody (orb1258453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of MCF-7 cells using EIF6 antibody (orb1258453). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using EIF6 antibody (orb1258453) at dilution of 1:50. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using EIF6 antibody (orb1258453) at dilution of 1:50. Blue: DAPI for nuclear staining.