EIF4B Antibody

• Catalog Number: orb1257507
• Category: Antibodies
• Description: EIF4B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 80kDa
• Target: EIF4B
• UniProt ID: P23588
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: EIF4B, EIF-4B, PRO1843
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EIF4B antibody (orb1257507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using EIF4B antibody (orb1257507). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug EIF4B antibody (orb1257507). Western blot was performed from the immunoprecipitate using EIF4B antibody (orb1257507) at a dilution of 1:1000.