EIF4B Antibody

SKU: orb1257507

Description

EIF4B Antibody

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
• Target: EIF4B
• Molecular Weight: Observed: 80kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

EIF4B, EIF-4B, PRO1843

Western blot analysis of extracts of various cell lines, using EIF4B antibody (orb1257507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using EIF4B antibody (orb1257507). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug EIF4B antibody (orb1257507). Western blot was performed from the immunoprecipitate using EIF4B antibody (orb1257507) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

EIF4B

UniProt

P23588