EIF4B Antibody

• Catalog Number: orb97470
• Category: Antibodies
• Description: Mouse monoclonal antibody to EIF4B
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1F5D7
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2b
• Immunogen: Purified recombinant fragment of human EIF4B expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 80kDa
• Target: EIF4B
• Entrez: 1975
• UniProt ID: P23588
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: EIF-4B; PRO1843
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EIF4B antibody

Western blot analysis of human (AA: 381-585) recombinant protein using EIF4B antibody

Western blot analysis of A549 (Lane1), A431 (Lane2), HepG2 (Lane3), HEK293 (Lane4), HeLa (Lane5), Jurkat (Lane6), K562 (Lane7), NIH3T3 (Lane8), and MCF-7 (Lane9) cell lysate using EIF4B antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded colon cancer tissues using EIF4B antibody

Flow cytometric analysis of HeLa cells using EIF4B antibody

Confocal immunofluorescence analysis of NIH3T3 cells using EIF4B antibody