EIF4B Antibody

SKU: orb97470

Description

Mouse monoclonal antibody to EIF4B

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: 1F5D7
• Immunogen: Purified recombinant fragment of human EIF4B expressed in E. Coli.
• Target: EIF4B
• Molecular Weight: 80kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

EIF-4B; PRO1843

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EIF4B antibody

Western blot analysis of human (AA: 381-585) recombinant protein using EIF4B antibody

Western blot analysis of A549 (Lane1), A431 (Lane2), HepG2 (Lane3), HEK293 (Lane4), HeLa (Lane5), Jurkat (Lane6), K562 (Lane7), NIH3T3 (Lane8), and MCF-7 (Lane9) cell lysate using EIF4B antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded colon cancer tissues using EIF4B antibody

Flow cytometric analysis of HeLa cells using EIF4B antibody

Confocal immunofluorescence analysis of NIH3T3 cells using EIF4B antibody

Quick Database Links

Gene Symbol

EIF4B

UniProt

P23588

NCBI Gene ID

1975