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Catalog Number | orb1262327 |
---|---|
Category | Antibodies |
Description | EIF4A2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Bovine, Gallus, Monkey, Mouse, Rabbit, Rat |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human EIF4A2. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 46 kDa |
Target | EIF4A2 |
UniProt ID | Q14240 |
NCBI | Q14240 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Eukaryotic initiation factor 4A-II, eIF-4A-II, eIF Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of EIF4A2 using rabbit polyclonal EIF4A2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |