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EIF4A2 Antibody
SKU: orb1262327
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Description
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−Item 1 of 5
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Gallus, Monkey, Mouse, Rabbit, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human EIF4A2. |
| Target | EIF4A2 |
| Molecular Weight | 46 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Eukaryotic initiation factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA helicase eIF4A-2, EIF4A2, DDX2B, EIF4F
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Western Blot at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of EIF4A2 using rabbit polyclonal EIF4A2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
EIF4A2 Antibody (orb1262327)
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