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EIF4A2 Antibody

SKU: orb1257810

Description

EIF4A2 Antibody

Images & Validation

Tested ApplicationsIHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:1000IHC: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
TargetEIF4A2
Molecular WeightObserved: 46kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

EIF4A2, DDX2B, EIF4A, EIF4F, BM-010, eIF4A-II, eIF-4A-II

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EIF4A2 Antibody

Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (orb1257810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

EIF4A2 Antibody

Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).

EIF4A2 Antibody

Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).

EIF4A2 Antibody

Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol

EIF4A2 Antibody (orb1257810)

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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