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Catalog Number | orb1257810 |
---|---|
Category | Antibodies |
Description | EIF4A2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 46kDa |
Target | EIF4A2 |
UniProt ID | Q14240 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | EIF4A2, DDX2B, EIF4A, EIF4F, BM-010, eIF4A-II, eIF Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (orb1257810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Gallus, Monkey, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |