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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 694-913 of human EIF3C (NP_003743.1). |
| Molecular Weight | 105kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti EIF3CL antibody, anti EIF3S8 antibody, anti eIF3-p110 antibody
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Immunofluorescence analysis of U2OS cells using EIF3C Rabbit pAb (orb247746). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using EIF3C Rabbit pAb (orb247746) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded Mouse intestine using EIF3C Rabbit pAb (orb247746) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded Rat brain using EIF3C Rabbit pAb (orb247746) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western blot analysis of various lysates using EIF3C Rabbit pAb (orb247746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Keck, Michael et al. A systems level analysis of epileptogenesis-associated proteome alterations Neurobiol Dis, 105, 164-178 (2017)
EIF3C Rabbit pAb (orb247746)
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