EIF3CL Antibody (N-term)

• Catalog Number: orb1937332
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This EIF3CL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-155 amino acids from the N-terminal region of human EIF3CL.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q99613
• MW: 105344 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB32067
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Eukaryotic translation initiation factor 3 subunit
• Note: For research use only
• NCBI: NP_001032897.1, NP_001254503.1, NP_001186071.1, NP_003743.1

EIF3CL Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-EIF3CL Antibody (N-term) at 1:1000 dilution. Lane 1: K562 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

EIF3CL Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of EIF3CL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.