EIF2C1 Antibody

SKU: orb1247567

Description

EIF2C1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing Human EIF2C1bands of approx. 110kDa and 35kDa are observed. The 110kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2kDa according to NP_036331.1. Recommended concentration, 0.3-1µg/mL.Immunohistochemistry:Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 2.5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KNASYNLDPYIQEF
• Target: EIF2C1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EIF2C1, eukaryotic translation initiation factor 2C, 1, AGO1, EIF2C, GERP95, Q99 , Golgi Endoplasmic Reticulum protein 95 kDa, argonaute 1, putative RNA-binding protein Q99

HEK293 overexpressing EIF2C1 and probed with orb1247567 (non-transfected HEK293 in lane B).

orb1247567 (2.5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247567 (2.5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

EIF2C1

UniProt

Q9UL18

RefSeq (Protein):NP_036331.1