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Anti-EEF2 Antibody

Catalog Number: orb692171

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb692171
CategoryAntibodies
DescriptionAnti-EEF2 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Zebrafish.
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Monkey, Mouse, Rat, Zebrafish
Form/AppearanceLyophilized
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
ImmunogenE.coli-derived human EEF2 recombinant protein (Position: M1-L858).
UniProt IDP13639
MW95 kDa
Tested applicationsELISA, IHC, WB
Application notesWestern blot, 0.1-0.25μg/ml, Human, Monkey, Mouse, Rat, Zebrafish Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500μg/ml
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesProtein argonaute-1; Argonaute1; hAgo1; Argonaute
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-EEF2 Antibody

IHC analysis of EEF2 using anti-EEF2 antibody. EEF2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-EEF2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-EEF2 Antibody

IHC analysis of EEF2 using anti-EEF2 antibody. EEF2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-EEF2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-EEF2 Antibody

IHC analysis of EEF2 using anti-EEF2 antibody. EEF2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-EEF2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-EEF2 Antibody

IHC analysis of EEF2 using anti-EEF2 antibody. EEF2 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-EEF2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-EEF2 Antibody

IHC analysis of EEF2 using anti-EEF2 antibody. EEF2 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-EEF2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-EEF2 Antibody

IHC analysis of EEF2 using anti-EEF2 antibody. EEF2 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-EEF2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-EEF2 Antibody

Western blot analysis of EEF2 using anti-EEF2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: zebrafish tissue lysates, Lane 9: rat stomach tissue lysates, Lane 10: rat pancrease tissue lysates, Lane 11: rat C6 whole cell lysates, Lane 12: mouse pancrease tissue lysates, Lane 13: mouse 3T3L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2 antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EEF2 at approximately 95 kDa. The expected band size for EEF2 is at 95 kDa.

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