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EGR2 Antibody

SKU: orb1247570

Description

EGR2 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 55kDa band observed in lysates of cell line HepG2 (calculated MW of 50.3kDa according to NP_000390.2). Recommended concentration: 2-6µg/mL. Primary incubation was 1 hour.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: HGTAGPDRKPFPC
TargetEGR2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

EGR2, early growth response 2 (Krox-20 homolog, Drosophila) , CMT1D, CMT4E, DKFZp686J1957, FLJ14547, KROX20, KROX-20, Drosophila, homolog (early growth response-2), Krox-20 homolog, OTTHUMP00000062133 , early growth response 2 protein

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

EGR2 Antibody

orb1247570 (2 ug/ml) staining of lysates of cell line HepG2 (35 ug protein in RIPA buffer). Detected by chemiluminescence.

EGR2 Antibody

orb1247570 (3.8 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

EGR2 Antibody

orb1247570 (3.8 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

EGR2 Antibody (orb1247570)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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