EGFR Antibody (Y869)

• Catalog Number: orb1929070
• Category: Antibodies
• Description: EGFR Antibody (Y869)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P00533
• MW: 134277 Da
• Tested applications: WB
• Dilution range: WB - 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ERBB, ERBB1, HER1
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGFR (arrow) using rabbit polyclonal EGFR Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EGFR gene (Lane 2).

All lanes: Anti-EGFR Antibody (Y869) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.