EGFR (Phospho-Y869) Antibody

• Catalog Number: orb1289870
• Category: Antibodies
• Description: The EGFR (Phospho-Y869) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y869 of human EGFR protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y869 of human EGFR protein. The exact sequence is proprietary.
• UniProt ID: P00533
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1:1000, IF:IC: 1:50-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB; ERBB1; HER1; Epidermal growth factor recepto
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Entrez: 1956
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGFR (Phospho-Y869) expression in Hela (A) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)

Immunofluorescent analysis of EGFR (Phospho-Y869) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.