EGFR Antibody

• Catalog Number: orb395002
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGFR-Specific.
• Target: EGFR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Immunogen: epidermal growth factor receptor(erythroblastic leukemia viral(v-erb-b) oncogene homolog, avian)
• UniProt ID: P00533
• MW: 145-165 kDa
• Tested applications: ELISA, IF, IHC, IP, WB
• Dilution range: WB: 1:2000-1:4000; IP: 1:500-1:1000; IHC: 1:200-1:800; IF: 1:50-1:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of A431 cells using orb395002 (EGFR-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-EGFR-Specific (IP: orb395002, 3 ug; Detection: orb395002 1:500) with MCF-7 cells lysate 2500 ug.

L02 cells were subjected to SDS PAGE followed by western blot with orb395002 (EGFR-Specific antibody) at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395002 (EGFR-Specific antibody) at dilution of 1:400.