EGFR Specific Rabbit Polyclonal Antibody

SKU: orb395002

Description

Rabbit polyclonal antibody to EGFR-Specific.

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: WB: 1:2000-1:4000; IP: 1:500-1:1000; IHC: 1:200-1:800; IF: 1:50-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: epidermal growth factor receptor(erythroblastic leukemia viral(v-erb-b) oncogene homolog, avian)
• Target: EGFR
• Molecular Weight: 145-165 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent analysis of A431 cells using orb395002 (EGFR-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-EGFR-Specific (IP: orb395002, 3 ug; Detection: orb395002 1:500) with MCF-7 cells lysate 2500 ug.

L02 cells were subjected to SDS PAGE followed by western blot with orb395002 (EGFR-Specific antibody) at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395002 (EGFR-Specific antibody) at dilution of 1:400.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533