EGF R Antibody

SKU: orb1252778

Description

EGF R Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  IHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 µg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: GFR450
• Immunogen: The immunizing protein for this antibody was recombinant human EGFR.
• Target: EGF R
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

IHC testing of squamous cell carcinoma stained with EGFR antibody (GFR450).

FACS testing of A431 cells with isotype control (gray) and EGFR antibody (green).

Immunofluorescent staining of A431 cells with Alexa Fluor conjugated EGFR antibody (green) and DAPI (nuclear, blue).

SDS-PAGE Analysis of Purified, BSA-Free EGFR Antibody (clone GFR450). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

EGF R

UniProt

P00533