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Item 1 of 4
Item 1 of 4
Anti-Hu EGFR (pY992) Purified
Catalog Number: orb43774
| Catalog Number | orb43774 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to EGFR (phospho-Tyr992). |
| Clonality | Monoclonal |
| Clone Number | EM-12 |
| Tested applications | FC, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | EGFR (pY992) |
| Entrez | 1956 |
| UniProt ID | P00533 |
| RRID | AB_10996081 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-EGFR (Phospho-Tyr992) antibody Read more... |
| Note | For research use only |
| Application notes | Western blotting: Recommended dilution: 1 μg/ml; positive control: EGF-stimulated A431, negative control: non-stimulated A431. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining. |
| Expiration Date | 12 months from date of receipt. |
Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.
Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).
Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY992) (EM-12) purified antibody (concentration in sample 1 µg/ml, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining).
