EGF R Antibody

SKU: orb1252103

Description

EGF R Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC
• Reactivity: Human
• Application Notes:
  IHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: ERB59-1
• Immunogen: Recombinant human protein was used as the immunogen for this EGFR antibody.
• Target: EGF R
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green) with DAPI nuclear stain (blue).

IHC testing of lung squamous cell carcinoma stained with EGFR antibody (ERB59-1).

FACS testing of A431 cells with isotype control (gray) and EGFR antibody (green).

Quick Database Links

Gene Symbol

EGF R

UniProt

P00533