EGF Receptor Antibody

SKU: orb749351

Description

This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa-induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor.EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: Blocking, FACS, IP, NeA, Purification
• Dilution Range: Affinity purification (order BSA/sodium azide-free format for coupling),Blocks ligand-induced activation of EGFR,Inhibits proliferation of A431 cells (order BSA/azide-free format),Arrests tumor growth in vivo (order BSA/azide-free format),Immunoprecipitation: 1-2ug/500ug protein lysate,Flow cytometry: 0.5-1ug/10^6 cells
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: B1D8
• Immunogen: Microsomes from A431 cells were used as the immunogen for this antibody.
• Purification: Protein G purified monoclonal antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only