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EGF Antibody

SKU: orb1535465

Description

EGF Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
ReactivityHuman, Monkey, Rabbit
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 160kD double band observed in Human Prostate lysates (calculated MW of 134kD according to NP_001954.2). The two bands represent glycosylated forms of the immature protein. Recommended concentration: 1-3 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence SRQERVCNIEKNVS, from the internal region (near the N-Terminus) of the protein sequence according to NP_001954.2NP_001171601.1NP_001171602.1.
TargetEGF
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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50 μg
$ 740.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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