Egf Antibody

SKU: orb1272685

Description

Egf Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Rat
• Application Notes:
  Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/mL), a concentration of ELISA:Indirect:To detect Rat EGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.SandwichTo detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF. Western Blot:To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Rat EGF specific antibody was purified by affinity chromatography employing immobilized Rat EGF matrix.
• Target: Egf
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pro-epidermal growth factorEGF

To detect Rat EGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF (orb1272684) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.

To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected rat brain (mammillary nuclei) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

Egf

UniProt

P07522

RefSeq:P07522