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Egf Antibody

SKU: orb1272569

Description

Egf Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityMouse
Application Notes
ELISA:To detect murine EGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine EGF. Western Blot:To detect murine EGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenProduced from sera of goats pre-immunized with highly pure (>98%) recombinant murine EGF.
TargetEgf
PurificationAnti-murine EGF specific antibody was purified by affinity chromatography employing immobilized murine EGF matrix.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

AI790464Pro-epidermal growth factorEGF

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP01132

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 780.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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