EDF1 Antibody

SKU: orb1248331

Description

EDF1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NKQHSITKNTAKLDR
• Target: EDF1
• Molecular Weight: Approx 17kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

EDF1, endothelial differentiation-related factor 1, EDF-1, MBF1, multiprotein bridging factor 1

orb1248331 (0.3 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248331 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

EDF1

UniProt

O60869

RefSeq (Protein):NP_003783.1, NP_694880.1