EDF1/MBF1 Antibody

SKU: orb570253

Description

Goat polyclonal antibody to EDF1

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  WB: Approx 19kDa band observed in nuckear lysates of cell line HEK293, approx. 18kDa in nuckear lysates of cell line HeLa, and approx 17kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4kDa according to Human NP_003783.1 and Mouse NP_067494.1).. IHC: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: EDF1 / MBF1
• Molecular Weight: 16.4; 15.5; 15.6; 14.1; 14.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

EDF1; MBF1; EDF-1; MGC9058; endothelial differentiation-related factor 1; multiprotein bridging factor 1

Flow cytometric analysis of A431 cells using EDF1 antibody.

Immunofluorescence analysis of HeLa cells using EDF1 antibody.

Immunofluorescence analysis of A431 cells using EDF1 antibody.

Western blot analysis of HEK293 (lane 1), HeLa (lane 2) and NIH3T3 (lane 3) Nuclear cell lysate using EDF1 antibody.

Immunohistochemical staining of Human Cortex of EDF1 antibody

Immunohistochemical staining of Human Pancreas of EDF1 antibody.

Quick Database Links

Gene Symbol

EDF1 / MBF1

NCBI Gene ID

8721