Goat anti-EDF1 / MBF1 Antibody

• Catalog Number: orb570253
• Category: Antibodies
• Description: Goat polyclonal antibody to EDF1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 16.4; 15.5; 15.6; 14.1; 14.4
• Target: EDF1 / MBF1
• Entrez: 8721
• Protein Sequence: AESDWDTVTVLRK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: EDF1; MBF1; EDF-1; MGC9058; endothelial differenti
• Note: For research use only
• Application notes: WB: Approx 19kDa band observed in nuckear lysates of cell line HEK293, approx. 18kDa in nuckear lysates of cell line HeLa, and approx 17kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4kDa according to Human NP_003783.1 and Mouse NP_067494.1).. IHC: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of A431 cells using EDF1 antibody.

Immunofluorescence analysis of HeLa cells using EDF1 antibody.

Immunofluorescence analysis of A431 cells using EDF1 antibody.

Western blot analysis of HEK293 (lane 1), HeLa (lane 2) and NIH3T3 (lane 3) Nuclear cell lysate using EDF1 antibody.

Immunohistochemical staining of Human Cortex of EDF1 antibody

Immunohistochemical staining of Human Pancreas of EDF1 antibody.