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E.coli Combined HCP Antibody

SKU: orb344701

Description

E.coli Combined HCP antibody

Research Area

Infectious Disease & Virology

Images & Validation

Tested Applications2D-PAGE, ELISA, WB
Dilution RangeELISA: 1:1000-1:2000, WB: 1:500-1:1000
ReactivityE. coli
Application Notes
Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by 2D-Page, western blotting, and ELISA.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.coli (BL21).
PurityHost Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration0.82 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

Escherichia coli Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit anti-E.coli HCP antibody

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Quality Guarantee

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

25 μl
Free Secondary Antibody (20 ul)0/0

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