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Catalog Number | orb344701 |
---|---|
Category | Antibodies |
Description | E.coli Combined HCP antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | 2D-PAGE, ELISA, WB |
Reactivity | E. coli |
Isotype | IgG |
Immunogen | Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21). |
Concentration | 0.82 mg/mL |
Dilution range | ELISA: 1:1000-1:2000, WB: 1:500-1:1000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known. |
Conjugation | Unconjugated |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Host Cell Protein antibody, rabbit ant Read more... |
Note | For research use only |
Application notes | Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by 2D-Page, western blotting, and ELISA. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of E.coli Host Cell Protein using E.coli Combined HCP antibody
2D-PAGE, ELISA, WB | |
E. coli | |
Rabbit | |
Polyclonal | |
Unconjugated |
2D-PAGE, ELISA, WB | |
E. coli | |
Rabbit | |
Polyclonal | |
Unconjugated |
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