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Description
Research Area
Infectious Disease & Virology
Images & Validation
−| Tested Applications | 2D-PAGE, ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:1000-1:2000, WB: 1:500-1:1000 |
| Reactivity | E. coli |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.coli (BL21). |
| Purity | Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.02mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−Escherichia coli Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit anti-E.coli HCP antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)









