EBI3 Antibody

• Catalog Number: orb1238976
• Category: Antibodies
• Description: EBI3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-EBI-3 antibody (orb1238976) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The immunogen is located within the last 50 amino acids of EBI3.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-4 μg/mL; IF: 20 μg/mL ; IHC: 2.5 μg/mL;.Antibody validated: Western Blot, Immunofluorescence, and Immunohistochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 25kDObserved: 30 kD (25kD + 2 N-Glycosylations)
• Target: EBI3
• UniProt ID: Q14213
• NCBI: NP_005746
• Storage: EBI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane. Antibodies: EBI-3 orb1238976, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL, Lane 2: 2 µg/mL, Lane 3: 4 µg/mL.

Immunohistochemistry Validation of EBI-3 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded Human Spleen tissue using anti-EBI-3 antibody (orb1238976) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of EBI-3 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen tissue labeling EBI-3 with orb1238976 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).