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Validated
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IHC-P, IP, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | BT25X7I5 |
| Immunogen | A synthetic peptide of human E2F1 |
| Target | E2F1 |
| Molecular Weight | Calculated MW: 47 kDa; Observed MW: 70 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% rAlbumin |
| Concentration | 0.3 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of E2F1 in MOLT4 lysates using E2F1 antibody.

Western blot analysis of E2F1 in C6, 3T3 lysates using E2F1 antibody.

Immunocytochemistry analysis of E2F1 (green) in Hela using E2F1 antibody, and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded Human tonsil using E2F1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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E2F1 Antibody (orb1563068)
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