E2F1 Antibody

SKU: orb97489

Description

Mouse monoclonal antibody to E2F1

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 8G9B2
• Immunogen: Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.
• Target: E2F1
• Molecular Weight: 46.9kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

RBP3; E2F-1; RBAP1; RBBP3

IHC-P of rectum cancer tissues using E2F1 antibody

Western blot analysis of human recombinant protein using E2F1 antibody

Flow cytometric analysis of HeLa cells using E2F1 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded esophageal cancer tissues using E2F1 antibody

Western blot analysis of HeLa (Lane1), SK-N-SH (Lane2), and NIH3T3 (Lane3) cell lysate using E2F1 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of E2F1 antibody

Quick Database Links

Gene Symbol

E2F1

UniProt

Q01094

NCBI Gene ID

1869