E-Cadherin Antibody / CDH1

• Catalog Number: orb699729
• Category: Antibodies
• Description: E-cadherin is a transmembrane, calcium dependent cell adhesion protein that mediates cell to cell adhesion and maintains structural and functional integrity of epithelial tissues. It also has pivotal barrier functions and maintains the polarity of epithelial cells. Reduced or aberrant E-cadherin expression breaks cell to cell contacts, and thus, cells acquire the ability to migrate. In normal tissues, immunostaining of E-cadherin is localized to the membrane of epithelial cells, consistent with its role in cell adhesion. And in tumor tissues, E-cadherin stains positively in glandular epithelium as well as adenocarcinomas of the lung, gastrointestinal tract, and ovary. It has also been shown to be positive in some thyroid carcinomas. A combination of E-cadherin and p120 catenin may help distinguish ductal carcinoma of the breast from lobular carcinoma. And also, several studies have reported that reduced E-cadherin expression is correlated with poor prognosis in several types of carcinomas.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CDH1/4398R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 600-700 from the human protein was used as the immunogen for the recombinant E-Cadherin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant E-Cadherin antibody is available for research use only.
• UniProt ID: P12830
• Storage: Store the recombinant E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant E-Cadherin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.