DYX1C1 Antibody

• Catalog Number: orb1247579
• Category: Antibodies
• Description: DYX1C1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KIRNVIQGTELKS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1ug/ml antibody staining. In addition, a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA). The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells. Recommended concentration, 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DYX1C1
• UniProt ID: Q8WXU2
• NCBI: NP_570722.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DYX1C1, DYX1, EKN1, DYXC1, FLJ37882, dyslexia susc
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1ug/ml antibody staining. In addition, a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA). The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247579 staining (0.1 ug/ml) of COS1 cell lysates: untransfected (left lane) and transfected with full length recombinant Human DYX1C1 (right lane).

orb1247579 (5 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.