DYX1C1 (Isoform a) Antibody

• Catalog Number: orb19001
• Category: Antibodies
• Description: Goat polyclonal antibody to DYX1C1
• Target: DYX1C1 (Isoform a)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KIRNVIQGTELKS
• RRID: AB_10749944
• MW: 48.5
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1 μg/ml, IHC-P: 5-10µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1 μg/ml antibody staining. In addition, a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA). The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DYX1C1 antibody, anti DYX1 antibody, anti EKN
• Note: For research use only
• Entrez: 161582

Western blot analysis of COS1 cell lysates using DYX1C1 antibody

Immunohistochemical staining of Human Cerebral Cortex using DYX1C1 antibody