DYDC1 Antibody

• Catalog Number: orb1249179
• Category: Antibodies
• Description: DYDC1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-TLAEISDRYGAPN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:n transfected HEK293 transiently expressing Human DYDC1 (myc and DYKDDDDK tagged), a band of approx. 28kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. This 28kDa band is also observed in Human, Mouse and Rat Testis lysates. Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DYDC1
• UniProt ID: Q8WWB3
• NCBI: NP_620167.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DYDC1, DPY30 domain containing 1, DPY30D1, FLJ4392
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:n transfected HEK293 transiently expressing Human DYDC1 (myc and DYKDDDDK tagged), a band of approx. 28kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. This 28kDa band is also observed in Human, Mouse and Rat Testis lysates. Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

Lane A - orb1249179 (1 ug/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10 ug protein in RIPA buffer) Lane B - orb1249179 (0.1 ug/ml) staining of HEK293 mock-transfected lysate (10 ug protein in RIPA buffer).

orb1249179 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.