DYDC1 (aa142-154) Antibody

SKU: orb20489

Description

Goat polyclonal to DYDC1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 28kDa band in Rat Testis lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.9kDa according to Human NP_620167.1 and of 21.8kDa according to Rat XP_001066918.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DYDC1 (aa142-154)
• Molecular Weight: 20.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti DYDC1 antibody, anti DPY30 domain containing 1 antibody, anti DPY30D1 antibody, anti FLJ43920 antibody, anti bA36D19.5 antibody, anti BC019250 protein antibody, anti OTTHUMP00000019963 antibody

Western blot analysis of HEK293 overexpressing Human DYDC1 lysate (Lane1), HEK293 mock-transfected lysate (Lane2), HEK293 overexpressing Human DYDC1 lysate (Lane3) using DYDC1 antibody

Immunohistochemical staining of Human Testis using DYDC1 antibody

Quick Database Links

Gene Symbol

DYDC1 (aa142-154)

NCBI Gene ID

143241