DOK5 Antibody

SKU: orb1247597

Description

DOK5 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing DOK5 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5kDa according to NP_060901.2. Recommended concentration, 1-3ug/ml.Immunohistochemistry:In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells. Recommended concentration, 1-2ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CLHRTETFPAYRSEH
• Target: DOK5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

DOK5, MGC16926, C20orf180, docking protein 5, downstream of tyrosine kinase 5, chromosome 20 open reading frame 180

HEK293 overexpressing DOK5 and probed with orb1247597 (mock transfection in first lane).

orb1247597 (1 ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

Quick Database Links

Gene Symbol

DOK5

UniProt

Q9P104

RefSeq (Protein):NP_060901.2