DOK5 Antibody

SKU: orb18915

Description

Goat polyclonal to DOK5

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 1-2µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells. Recommended concentration, 1-2ug/ml.WB: In transfected HEK293 transiently expressing DOK5 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5kDa according to NP_060901.2. Recommended concentration, 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DOK5
• Molecular Weight: 35.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Disclaimer: For research use only

Alternative Names

anti DOK5 antibody, anti MGC16926 antibody, anti C20orf180 antibody, anti docking protein 5 antibody, anti downstream of tyrosine kinase 5 antibody, anti chromosome 20 open reading frame 180 antibody

HEK293 overexpressing DOK5 (RC211718) and probed with orb18915 (mock transfection in first lane), tested by Origene.

orb18915 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

Quick Database Links

Gene Symbol

DOK5

NCBI Gene ID

55816