Dog1 antibody (MSVA-201M)

• Catalog Number: orb2276584
• Category: Antibodies
• Description: Dog1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human DOG-1 protein fragment (aa 2-101) (exact sequence is proprietary)
• UniProt ID: Q5XX6
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Appendix: An at least moderate, predominantly membranous staining should be seen in Cajal cells in the muscular wall and a weak membranous staining should be seen in columnar epithelial cells in the basal part of the crypts of the appendix. Negative Control: Appendix: Staining should be absent in the majority of cells in the muscular wall and in epithelial cells located at the surface. Cellular Localization: Cell Surface and Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-201M
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Anoctamin 1; Calcium Activated Chloride Channel; D
• Note: For research use only

Parotid gland: Strong DOG1 staining of the apical membranes of secreting cells.

Seminal vesicle: Strong DOG1 staining of apical membranes of glandular cells.

Dog1 Tumor periphery of a GIST showing intensive DOG1 staining in all tumor cells.