Dog1 antibody

SKU: orb2276584

Description

Dog1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Appendix: An at least moderate, predominantly membranous staining should be seen in Cajal cells in the muscular wall and a weak membranous staining should be seen in columnar epithelial cells in the basal part of the crypts of the appendix. Negative Control: Appendix: Staining should be absent in the majority of cells in the muscular wall and in epithelial cells located at the surface. Cellular Localization: Cell Surface and Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: MSVA-201M
• Immunogen: Recombinant human DOG-1 protein fragment (aa 2-101) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anoctamin 1; Calcium Activated Chloride Channel; Discovered On Gastrointestinal Stromal Tumors Protein 1; TAOS2; ORAOV2; TMEM16A

Parotid gland: Strong DOG1 staining of the apical membranes of secreting cells.

Seminal vesicle: Strong DOG1 staining of apical membranes of glandular cells.

Dog1 Tumor periphery of a GIST showing intensive DOG1 staining in all tumor cells.

Quick Database Links

UniProt

Q5XX6