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| Catalog Number | orb1252254 |
|---|---|
| Category | Antibodies |
| Description | DOG1 Antibody [DG1/1484] |
| Target | ANO1 |
| Clonality | Monoclonal |
| Isotype | IgG2b, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | 0.2 mg/mL |
| Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Purification | Protein G affinity chromatography |
| Immunogen | A recombinant human protein corresponding to amino acids 2-101 of human DOG1/ANO1 was used as the immunogen for this DOG 1 antibody. |
| UniProt ID | Q5XXA6 |
| Tested applications | FC, IF, IHC-P, WB |
| Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG 1 antibody to be titered up or down for optimal performance. |
| Antibody Type | Primary Antibody |
| Clone Number | DG1/1484 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DO Read more... |
| Research Area | Signal Transduction |
| Note | For research use only |
![DOG1 Antibody [DG1/1484]](/images/pub/media/catalog/product/NewWebsite/15/orb1252254_1.jpg)
IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG 1 antibody (DG1/1484). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
![DOG1 Antibody [DG1/1484]](/images/pub/media/catalog/product/NewWebsite/15/orb1252254_2.jpg)
SDS-PAGE Analysis of Purified, BSA-Free DOG 1 Antibody (clone DG1/1484). Confirmation of Integrity and Purity of the Antibody.
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