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Catalog Number | orb346961 |
---|---|
Category | Antibodies |
Description | Dog IgG (H&L) antibody (Biotin) |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Biotin |
Reactivity | Canine |
Form/Appearance | Lyophilized |
Concentration | 2.0 mg/mL |
Buffer/Preservatives | 0.01% (w/v) Thimerosal |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Dog IgG and Dog Serum. |
Immunogen | Dog IgG whole molecule |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Application notes | This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:17,500 to 1:75,000 of the reconstitution concentration is suggested for this product. |
Antibody Type | Secondary Antibody |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Alternative names | rabbit anti Dog IgG Antibody Biotin Conjugated, ra Read more... |
Note | For research use only |
ELISA, IHC, WB | |
Canine | |
Rabbit | |
Polyclonal | |
Biotin |
ELISA, IH, WB | |
Rabbit | |
Polyclonal | |
Biotin |