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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000, IHC-P - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB01906 |
| Immunogen | This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b. Antigen Region: 389-417 aa. |
| Target | DNMT3B |
| Molecular Weight | 95751 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B
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All lanes: Anti-Dnmt3b Antibody (C403) at 1:2000 dilution. Lane 1: A431 whole cell lysates. Lane 2: A549 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 95 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Dnmt3b Antibody (C403) at 1:2000 dilution + T47D whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 95 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
Gene Symbol
DNMT3B
UniProt
RefSeq (Protein):NP_787046.1, NP_001193985.1, NP_787045.1, NP_787044.1, NP_008823.1, NP_001193984.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein productDocuments Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
Dnmt3b Antibody (orb1939027)
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