Dnmt3b antibody

• Catalog Number: orb372330
• Category: Antibodies
• Description: Dnmt3b antibody
• Target: Dnmt3b
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Immunogen: KLH conjugated synthetic peptide derived from human Dnmt3b. Please contact us for the exact immunogen sequence. The peptide is available as orb374989.
• UniProt ID: Q1LZ51, Q9UBC3, O88509
• MW: 96 kDa
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:100-500, IHC-P: 1:50-400
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ICF antibody, anti ICF1 antibody, anti M.HsaI
• Note: For research use only
• Entrez: 1789
• NCBI: 001193984, 001207055

References

da Silva, Rodrigo A. et al. Osteogenic gene markers are epigenetically reprogrammed during contractile-to-calcifying vascular smooth muscle cell phenotype transition Cell. Signal., 109458 (2019)

PubMed: 31678252

IHC-P staining of rat lung tissue using anti-Dnmt3b (dilution at 2.5 ug/ml)

IHC-P staining of rat lung tissue using Dnmt3b antibody (dilution at 2.5 ug/ml)

Western blot analysis of rat heart (lane 1), rat lung (lane 2), rat liver (lane 3), rat kidney (lane 4) using Dnmt3b antibody (1 ug/ml)

Immunohistochemical staining of pig small intestines tissue using anti-Dnmt3b (dilution of primary antibody - 2.5 ug/ml)

WB analysis of rat heart (lane 1), rat lung (lane 2), rat liver (lane 3), rat kidney (lane 4) using Dnmt3b antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded rat lung tissue using Dnmt3b antibody (primary antibody at 2.5 ug/ml)