DNMT1 Antibody

SKU: orb67387

Description

Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa

Research Area

Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC-P (1:1000)
• Reactivity: Fish, Human, Mouse, Zebrafish
• Application Notes:
  2 µg/ml of SMC-200 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 60B1220.1
• Immunogen: Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
• Target: DNMT1
• Molecular Weight: 180kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Magnification: 40x. ABC solution 1:1000 dilution, DAB solution 100-200uL/ slide.

Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:1000.

Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1. Tissue: medullar kidney tissue. Species: Mouse. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain.

Western Blot analysis of Human 293T cell lysates showing detection of ~180kDa DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1. Lane 1: Molecular Weight Marker. Lane 2: Human 293T Cell lysate. Load: 15 μg. Block: 1.5% BSA in 1X TBST. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 minutes at RT. Predicted/Observed Size: ~180kDa. Other Band (s): Lower bands are DNMT1 degradation.

Quick Database Links

Gene Symbol

DNMT1

UniProt

P26358

NCBI Gene ID

1786

RefSeq (Protein):NP_001370