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DNMT1 Antibody
SKU: orb1258696
Featured
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein of Human DNMT1. |
| Target | DNMT1 |
| Molecular Weight | Observed: 200kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1, AIM
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using DNMT1 antibody (orb1258696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
DNMT1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
DNMT1 Antibody (orb1258696)
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