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DNMT1 Antibody
Catalog Number: orb1258696
| Catalog Number | orb1258696 |
|---|---|
| Category | Antibodies |
| Description | DNMT1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein of Human DNMT1. |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 200kDa |
| Target | DNMT1 |
| UniProt ID | P26358 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using DNMT1 antibody (orb1258696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Goat anti-DNMT1 Antibody [orb19795]
ELISA, IHC, WB
Bovine, Canine, Human, Mouse, Porcine, Rat
Goat
Polyclonal
Unconjugated
100 μg - Item 1 of 1
Dnmt1 Rabbit Polyclonal Antibody [orb10546]
WB
Bovine, Canine, Equine, Gallus, Porcine, Sheep
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl
