DNMT1 Rabbit Polyclonal Antibody

SKU: orb626475

Description

Rabbit polyclonal antibody to DNMT1

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: DNA(cytosine-5-)-methyltransferase 1
• Target: DNMT1
• Molecular Weight: 180-200 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AIM, CXXC9, DNMT

Immunofluorescent analysis of HepG2 cells using orb626475 (DNMT1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

IP Result of anti-DNMT1 (IP: orb626475, 5 ug; Detection: orb626475 1:500) with HEK-293 cells lysate 2500 ug.

293T cells were subjected to SDS PAGE followed by western blot with orb626475 (DNMT1 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human breast cancer using orb626475 (DNMT1 antibody) at dilution of 1:100.

Quick Database Links

Gene Symbol

DNMT1

UniProt

P26358